Both the North and Southbound lanes by the Bridge Street interchange are being reduced from four to three, so crews can reconstruct the road between Carver and Levick Streets.
PennDOT's Brad Rudolph said work installing construction barriers and applying new road markings is expected to start Monday night, and run through Wednesday or Thursday.
"There will be a pattern in place where three throughlanes will get through, and everybody will be shifted to the southbound and the median," he explained.
Crews are setting up for a reconstruction project that will begin in late summer and continue for two years.
Rudolph added additional lanes may be closed overnight, but the on and off-ramps at Bridge Street will remain open.
"We're also going to be replacing the bridges over Van Kirk and Conley Streets, and this project also has a lot of neighborhood improvements and improvements on the ground," he said.
This is the second phase of the rebuild of I-95 between Center City and the Northeast.
Drivers are being encouraged to give themselves extra time to travel through the construction zone.